Molecular biology in Caserta
Laboratorio Sanitario San Carlo in Caserta carries out molecular biology examinations using state-of-the-art methodologies. Investigations are carried out in physically separate rooms, in a laboratory on the third floor of our building, so that sample handling operations - extraction, amplification and DNA detection, preparation of reaction mixes - do not run the risk of corruption and do not suffer any contamination.
The analytical methods carried out by our team are UNI CEI EN ISO/IEC 17025 validated and/or conducted following Good Laboratory Practice standards.
Instrumentation
The instrumentation dedicated to molecular biology investigations at Laboratorio Sanitario San Carlo in Caserta includes:
- 4 laminar flow hoods;
- 4 Samag automatic DNA extractors (12 and 24);
- 2 ROTOGENE Real Time Amplifiers;
- 4 SaCycler Real Time Amplifiers;
- digital amplification detection;
- Dubnoff baths for Reveverse Dot Blot;
- instrumentation for RFLP.
Diagnostic techniques
Laboratorio Sanitario San Carlo in Caserta, concerning molecular biology investigations, uses PCR, or Polymerase Chain Reaction. This is an in vitro diagnostic technique perfected by Kary Mullis in 1983, which, through exponential amplification of nucleic acids, makes it possible to identify human, bacterial, viral and protozoan DNA or RNA sequences with very high sensitivity and precision.
Real Time PCR, on the other hand, is a kinetic technique that allows a fluorescent molecule to be introduced into the reaction so that the reaction can be followed visually using special software. Finally, PCR + Reverse Dot blot allows the extraction and purification of DNA on a column, but also the amplification and detection of target DNA on special nylon strips.
Rapid diagnostic tests with state-of-the-art instruments
